Setting
WSL and FRP
Experiment
Method
- Cell
The degree of substitution was modulated as a function of ratio of DVS to OH, reaction time, and temperature. ^1
0.1g Dextran polymers were dissolved in 15mL DMSO
0.1/162*0.1*122.17=7.5mg DMAP was added
0.1/162*3*5*118.15g DVS was added
Reacted at dark environment for about 8hr
Precipitated in ether, than dissolved in DMSO
Precipitated in ethanol
x/(Mn of Dex-VS)*(Mn of Dex)/(Mn of one unit of Dex)*2=0.00875mmol
[^2]: Conditions vary with different kind of polypeptides